Withanolide-A Accelerates Re-entrainment of Circadian Locomotor Activity Rhythm and Preserves Spatial Memory in Mice Following Acute Phase Inversion of Light-Dark Cycles

Objective: Circadian rhythms are present almost in all living organisms and synchronized with the zeitgebers (time givers), especially lightdark (LD) cycles. The jetlag rotating shift work schedules lead to desynchronization of circadian clock which may cause various health problems. Studies have shown that withanolide-A (WA) is a potent nootropic neuroprotective agent found Withania somnifera. Thus, objective was study effect WA on locomotor activity rhythm following phase inversion 12:12 h LD cycle mice its impact spatial memory mice.Methods: Adult male were housed chronocubicle recorded by using ClockLab setup. experimental administered (10 μmol/kg/day) for 13 consecutive days at about ZT15 then evoked an acute light-dark cycle. Further, Barnes maze test performed check inversion.Results: results showed WA-treated group took significantly fewer transient cycles (4.66+0.66) re-entrainment than control (9.5+0.43) vehicle-treated (8.33+1.28) inversion. Similarly, traveled less distance reach target zone compared vehicle groups.Conclusion: These suggest act as chronobiotic since it decreased retaining